CMA Microdialysis AB

Exploring Tissue Chemistry

CMA Microdialysis has been the world’s leading microdialysis provider for more than 25 years. Microdialysis is a valuable tool for in vivo evaluation studies on drug delivery, drug metabolism, PK/PD, bioavailability, bioequivalence and pharmacological efficacy.

CMA´s vision is: To substantially shorten the time-to-market of drug and research projects by offering a complete and leading portfolio of Microdialysis products.

The principle of Microdialysis

The Microdialysis Probe mimics the function of a blood vessel. The probe is constantly perfused with a physiological solution at a low flow-rate (usually less then 2 μl/min). Once the probe is implanted into the tissue, endogenous substances are filtered by diffusion out of the extracellular fluid into the perfusion medium. By reversing the process the probe can be used to locally infuse exogenous compounds, nutrients and drugs for periods of up to several days. Samples are collected and then analyzed.

The Microdialysis Probe has a semipermeable membrane at its tip. A variety sizes and membranes are available depending on where you like to take samples. The inlet side of the Microdialysis Probe is connected to a CMA syringe pump. The outlet tubing is attached to a CMA Fraction Collector. Microdialysis combined with an appropriate analytical technique is the ultimate solution in the search for a universal, real-time biosensor.

Examples of where Microdialysis can be applied?

Mechanisms of drug action on release, uptake and interactions among neurotransmitters and neuromodulators represent the classical application field for Microdialysis. Neurochemical correlates to different models of mental disorder, behavioral and cognitive functions can be studied in chronically implanted freely moving animals.

Neuropathology and cancer research
Microdialysis is an excellent tool for monitoring the compounds proposed as markers of brain injury. Neurodegenerative diseases, such as ischaemia, hypoglycaemia and epilepsy, as well as, processes related to neuronal plasticity, regeneration, neurotransplantation or tumor growth have been elucidated by Microdialysis.

Pharmacokinetics and toxicology
Microdialysis probes can be implanted simultaneously in several organs (including blood) of the same animal. Distribution and time course of free drug (toxin) concentrations are measured in vivo. Pharmacokinetic data can be calculated using theoretical compartment models.

Physiological stimuli such as physical exercise, nutrition or stress alter anabolic and catabolic phases of cell biochemistry in peripheral tissues (e.g. muscle, fat). Microdialysis data can serveas a cumulative index of treatment-induced metabolic changes over long time periods in animals or even plants.

CMA Microdialysis AB
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