Sovicell

Sovicell develops state-of-the-art ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicology) products and offers assay development services that enable our customers to rapidly obtain accurate and reproducible pharmacokinetic data about their drug candidates or other test substances.

ADMET Kits
Sovicell provides ready-to-use test kits for ADMET (A: absorption, D: distribution, M: metabolism, E: excretion, T: toxicology) to support preclinical research programs in drug discovery. For pharmaceutical R&D, the Company’s products facilitate a shift from labor intensive “home-brew” approaches to fully automated, minimal labor, processes. This in turn provides increased flexibility, reduced cost and increased efficiency of the drug discovery process.

TRANSIL Technology Platform
Our TRANSIL technology platform, which is a novel bead based system, underpins our reliable membrane permeability and protein binding assays. The ready-to-use TRANSIL assay kits are essentially smart dialysis systems that use an immobilized biological matrix with a very large surface area. This allows both rapid equilibration of dialysate binding and fast downstream quantification via LC-MS/MS as there is no need to separate the compounds from the immobilized matrix. As the assays do not require filter or dialysis membranes they avoid issues of unspecific binding to these filters and provide a means for assessing the physical properties of both small molecules and peptides.

Trusted Partner
Sovicell has proved a trustworthy partner to the pharmaceutical and biotechnology industries over the past twenty years.
Located in Leipzig, Germany, the company has partners in the US, Japan, and India.

Products

Ready-to-use assay systems for DMPK and ADMET studies

  • Plasma Protein Binding

  • Brain Tissue Binding

  • Brain-to-Plasma Distribution

  • Microsomal Binding

  • Intestinal Absorption

Sovicell provides ready-to-use assay systems for drug discovery. Available as kits, the assays are used for early screening of drugability properties such as a drug’s capacity for oral absorption, its distribution in the body and into target organs e.g measurement of its availability in the brain, and its capacity of being eliminated in the liver. The kits are typically employed in DMPK (drug metabolism and pharmacokinetics) as well as ADME (absorption, distribution, metabolism and excretion) studies. However, they may also be used to assess the potential toxicity of food additives and chemicals released into the environment. All products were developed in partnership with our customers to meet the direct needs of the industry.

• Plasma Protein Binding

The plasma protein binding of drugs continues to be important for critical pharmacokinetic evaluations and in routine clinical monitoring of drugs. This is because for many drugs, the therapeutic and toxic response correlates better with the concentration of diffusible, unbound drug than with the total drug concentration. The new TRANSILXL Equilibrium Shift Assay provides a unique means of measuring plasma binding of peptide drugs and oligonucleotides, which supports half-life optimization of these drug classes.
Several methods exist for assessing the unbound fraction of a drug in plasma. Standard methods include dialysis, ultrafiltration, centrifugation and rapid dialysis with immobilized plasma proteins such as TRANSILXL AGP and TRANSILXL HSA Binding kits. Once the unbound fraction of the drug become very low and comprises only 1% of the total drug in plasma or even less, then all the mentioned methods become inaccurate mainly because of analytical problems such as detection limits, non-linear responses (LC-MS/MS), or limited compound purity (scintillation counting). To overcome these limitations, as well as common issues with low recovery or limited solubility, we offer the TRANSILXL High Sensitivity Binding kit.

• Brain Tissue Binding

A fast high throughput Brain Absorption assay for estimation of brain tissue binding of drugs and the prediction of their disposition into the brain.
The assay is based on the affinity of compounds to brain membranes and provides an accurate estimation of the unbound fraction of drugs in brain and the brain-to-plasma distribution.

Features

  • Ready-to-use 96-well microtiter plates carrying TRANSIL beads with a single lipid bilayer reconstituted from porcine brain lipids, designed to conveniently measure brain tissue binding and predicts blood-brain barrier penetration.
  • Includes spreadsheet, facilitating manipulation of statistical parameters and calculation of final results.
  • Designed for automated liquid handlers or manual pipetting.

 

Benefits

  • TRANSIL bead s are integrated into a 96-well microtiter plates enable easy handling.
  • One plate can be used for measuring brain tissue binding and brain disposition of up to 12 compounds.
  • Only 12 minutes assay incubation time.
  • Rapid compound quantification due to immobilized plasma proteins (e.g. injection via RapidFire™).
  • Highly reproducible results and robust correlation with conventional dialysis technique.

• Brain-to-Plasma Distribution

A fast high throughput Brain Absorption assay for estimation of brain tissue binding of drugs and the prediction of their disposition into the brain.
The assay is based on the affinity of compounds to brain membranes and provides an accurate estimation of the unbound fraction of drugs in brain and the brain-to-plasma distribution.

Features

  • Ready-to-use 96-well microtiter plates carrying TRANSIL beads with a single lipid bilayer reconstituted from porcine brain lipids, designed to conveniently measure brain tissue binding and predicts blood-brain barrier penetration.
  • Includes spreadsheet, facilitating manipulation of statistical parameters and calculation of final results.
  • Designed for automated liquid handlers or manual pipetting.

Benefits

  • TRANSIL bead s are integrated into a 96-well microtiter plates enable easy handling.
  • One plate can be used for measuring brain tissue binding and brain disposition of up to 12 compounds.
  • Only 12 minutes assay incubation time.
  • Rapid compound quantification due to immobilized plasma proteins (e.g. injection via RapidFire™).
  • Highly reproducible results and robust correlation with conventional dialysis technique.

• Microsomal Binding

A fast high throughput assay for liver microsomal binding. Available as a kit, the assay is based on the affinity to human liver microsomal membranes.

Features

  • Ready-to-use 96-well microtiter plates carrying TRANSIL beads with a single lipid bilayer reconstituted from synthetic lipids matching the microsomal membrane composition, designed to conveniently measure microsomal binding.
  • Kit includes spreadsheet, facilitating manipulation of statistical parameters and calculation of final results.
  • Designed for automated liquid handlers or manual pipetting.

Benefits

  • TRANSIL beads are integrated into 96-well microtiter plates to enable easy handling.
  • One plate can be used for measuring microsomal binding of up to 12 compounds.
  • Only 12 minutes assay incubation time.
  • Rapid compound quantification due to immobilized plasma proteins (e.g. injection via RapidFire™).
  • Highly reproducible results and robust correlation with conventional dialysis technique.

• Intestinal Absorption

A ready-to-use liposome-based kit for rapid in vitro assessment of the ability of drugs to cross the intestinal epithelium.

Features

  • Ready-to-use 96-well microtiter plates carrying TRANSIL beads with a single lipid bilayer reconstituted from phosphatidyl choline, designed to conveniently measure membrane binding and membrane permeability.
  • Kit includes spreadsheet, facilitating manipulation of statistical parameters and calculation of final results and a prediction of the volume of distribution (Vss).
  • Designed for automated liquid handlers or manual pipetting.

Benefits

  • TRANSIL beads are integrated into 96-well microtiter plates to enable easy handling.
  • One plate can be used for measuring membrane binding and membrane permeability of up to 12 compounds.
  • Only 12 minutes assay incubation time.
  • Rapid compound quantification due to immobilized plasma proteins (e.g. injection via RapidFire™).
  • Highly reproducible results and robust correlation with conventional dialysis technique.
Deutscher Platz 5b, 04103 Leipzig
+49 341 520440
http://www.sovicell.com